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Image Search Results
Journal:
Article Title: Studies on transcriptional regulation of the mucosal T-cell integrin ?E?7 (CD103)
doi: 10.1046/j.1365-2567.2001.01232.x
Figure Lengend Snippet: Stimulation of transcription and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.
Article Snippet: The transcription start sites were identified by
Techniques: Expressing, Staining, Negative Control, Synthesized, Cell Culture, Hybridization
Journal:
Article Title: Studies on transcriptional regulation of the mucosal T-cell integrin ?E?7 (CD103)
doi: 10.1046/j.1365-2567.2001.01232.x
Figure Lengend Snippet: Stimulation of transcription and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.
Article Snippet: The transcription start sites were identified by RNA Ligase-Mediated Rapid Amplification of
Techniques: Expressing, Staining, Negative Control, Synthesized, Cell Culture, Hybridization